Recombinant Monoclonal Rabbit anti‑All species Biotin Antibody (clone BTN/2032R, Azide‑free,Carrier‑free, IHC) LS‑C751507
Recombinant Monoclonal Rabbit anti‑All species Biotin Antibody (clone BTN/2032R, Azide‑free,Carrier‑free, IHC) LS‑C751507
Antibody:
Biotin Rabbit Recombinant Monoclonal (Azide-free, Carrier-free) (BTN/2032R) Antibody
Format:
Unconjugated, Azide-free, Carrier-free
Overview
Antibody:
Biotin Rabbit Recombinant Monoclonal (Azide-free, Carrier-free) (BTN/2032R) Antibody
Format:
Unconjugated, Azide-free, Carrier-free
Specifications
Description
Biotin antibody LS-C751507 is an unconjugated rabbit recombinant monoclonal antibody to all species Biotin. Validated for IHC and ISH.
Target
All species Biotin
Clonality
IgG
Recombinant Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Azide-free, Carrier-free.
Also available
Unmodified.
Immunogen
Biotinylated sheep immunoglobulin. Antigen Molecular Weight: 244Da
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Applications
- IHC - Paraffin (0.5 - 1 µg/ml)
- In situ hybridization
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Flow Cytometry (0.5-1 ug/million cells) - Immunofluorescence (0.5-1 ug/ml) - In situ Nucleic Acid Hybridization; Western Blotting; Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.) - Optimal dilution for a specific application should be determined.
Storage
Store at -20°C to -80°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Applications:
Immunofluorescence, Flow Cytometry, ELISA
Species:
Applications:
IHC, Western blot, ELISA
Species:
Applications:
Western blot, Immunoprecipitation, ELISA
Species:
Applications:
Immunofluorescence, Flow Cytometry
Species:
Applications:
IHC, Western blot, ELISA
Species:
Applications:
ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/4/2025