Biotin

Mouse

IgG1 Monoclonal

SPM375

Unconjugated

Protein G purified

Azide-free, BSA-free. Also available Unmodified.

Biotinylated sheep immunoglobulin.

Biotin

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Western blot (1 - 3 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.