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| Catalog Number | Size | Price |
|---|---|---|
| LS-C4218-100 | 100 µg (1 mg/ml) | $572 |
Polyclonal Rabbit anti‑Human BRAF / B‑Raf Antibody (aa754‑765, WB) LS‑C4218
Polyclonal Rabbit anti‑Human BRAF / B‑Raf Antibody (aa754‑765, WB) LS‑C4218
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
B-Raf antibody LS-C4218 is an unconjugated rabbit polyclonal antibody to human B-Raf (BRAF) (aa754-765). Validated for WB.
Target
Human BRAF / B-Raf
Synonyms
BRAF | 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-Terminus cysteine added for conjugation to KLH.
Epitope
aa754-765
Specificity
Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). .
Applications
- Western blot (1 - 2 µg/ml)
Usage
Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 ug/ml detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 ug.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About BRAF / B-Raf
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Featured Products
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse, Rat, Chicken, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
