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Customer Service: (800) 227-6666 / (650) 697-3600


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Catalog Number Size Price
LS-C662572-10 10 µg $318 
LS-C662572-100 100 µg $470 
CASP4 / Caspase 4 Antibody - IHC analysis of Caspase 4 using anti-Caspase 4 antibody. Caspase 4 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Caspase 4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CASP4 / Caspase 4 Antibody - IF analysis of Caspase 4 using anti-Caspase 4 antibody Caspase 4 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Caspase 4 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CASP4 / Caspase 4 Antibody - Western blot - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - Western blot - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - Flow Cytometry analysis of A431 cells using anti-Caspase 4 antibody. Overlay histogram showing A431 cells stained with anti-Caspase 4 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 4 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CASP4 / Caspase 4 Antibody - IHC analysis of Caspase 4 using anti-Caspase 4 antibody. Caspase 4 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Caspase 4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CASP4 / Caspase 4 Antibody - IF analysis of Caspase 4 using anti-Caspase 4 antibody Caspase 4 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Caspase 4 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CASP4 / Caspase 4 Antibody - Western blot - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - Western blot - Anti-Caspase 4 Picoband antibody
CASP4 / Caspase 4 Antibody - Flow Cytometry analysis of A431 cells using anti-Caspase 4 antibody. Overlay histogram showing A431 cells stained with anti-Caspase 4 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase 4 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Mouse CASP4 / Caspase 4 Antibody (WB) LS‑C662572

Polyclonal Rabbit anti‑Mouse CASP4 / Caspase 4 Antibody (WB) LS‑C662572

Antibody:
CASP4 / Caspase 4 Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662572-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CASP4 / Caspase 4 Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 4 antibody LS-C662572 is an unconjugated rabbit polyclonal antibody to mouse Caspase 4 (CASP4). Validated for ELISA and WB.
Target
Mouse CASP4 / Caspase 4
Synonyms
CASP4 | CASP-4 | Casp11 | Caspase 4 | Caspase-4 | Cysteine protease icerel-ii | ICE(rel)-II | ICE(rel)II | ICH-2 | Ich-3 | ICH2 | Protease ICH-2 | TX | Tx protease | Caspase 11 | ICEREL-II | Mih1/TX | Protease TX
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived mouse Caspase 4 recombinant protein (Position: E108-T195).
Specificity
Widely expressed, including in thymus, lung and spleen (at protein level). Very low levels, if any, in the brain.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP4 / Caspase 4
P49662 NM_001225 NP_001216.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/23/2024