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Antibodies > CASP8 / Caspase 8 > LS-C783060
 
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Catalog Number Size Price
LS-C783060-25 25 µg (0.5 mg/ml) $288 
LS-C783060-100 100 µg (0.5 mg/ml) $414 
CASP8 / Caspase 8 Antibody - Fig-5: Immunohistochemical analysis of Caspase-8 in human Tonsil using anti-Caspase-8 antibody at 5 µg/ml.
CASP8 / Caspase 8 Antibody - Fig-4: Immunohistochemical analysis of Caspase-8 in human Esophagus using anti-Caspase-8 antibody at 5 µg/ml.
CASP8 / Caspase 8 Antibody - Fig-6: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody was used at 4 µg/ml on EL-4 lysate.
CASP8 / Caspase 8 Antibody - Fig-1: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody was used at 2 µg/ml on Molt-4, Kato III and HepG2 lysates.
CASP8 / Caspase 8 Antibody - Fig-3: Intracellular Flow analysis of Caspase-8 antibody in Jurkat cells using 0.5 µg/ 10^6 cells of anti-Caspase-8 antibody. Green represents isotype control; red represents anti-Caspase-8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
CASP8 / Caspase 8 Antibody - Fig-2: Intracellular Flow analysis of Caspase-8 antibody in Hela cells using 0.5 µg/ 10^6 cells of anti-Caspase-8 antibody. Green represents isotype control; red represents anti-Caspase-8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
CASP8 / Caspase 8 Antibody - Fig-5: Immunohistochemical analysis of Caspase-8 in human Tonsil using anti-Caspase-8 antibody at 5 µg/ml.
CASP8 / Caspase 8 Antibody - Fig-4: Immunohistochemical analysis of Caspase-8 in human Esophagus using anti-Caspase-8 antibody at 5 µg/ml.
CASP8 / Caspase 8 Antibody - Fig-6: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody was used at 4 µg/ml on EL-4 lysate.
CASP8 / Caspase 8 Antibody - Fig-1: Western blot analysis of Caspase-8. Anti-Caspase-8 antibody was used at 2 µg/ml on Molt-4, Kato III and HepG2 lysates.
CASP8 / Caspase 8 Antibody - Fig-3: Intracellular Flow analysis of Caspase-8 antibody in Jurkat cells using 0.5 µg/ 10^6 cells of anti-Caspase-8 antibody. Green represents isotype control; red represents anti-Caspase-8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
CASP8 / Caspase 8 Antibody - Fig-2: Intracellular Flow analysis of Caspase-8 antibody in Hela cells using 0.5 µg/ 10^6 cells of anti-Caspase-8 antibody. Green represents isotype control; red represents anti-Caspase-8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).
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Monoclonal Mouse anti‑Human CASP8 / Caspase 8 Antibody (IHC, WB) LS‑C783060

Monoclonal Mouse anti‑Human CASP8 / Caspase 8 Antibody (IHC, WB) LS‑C783060

Submit Review LSBio Guarantee
Antibody:
CASP8 / Caspase 8 Mouse anti-Human Monoclonal Antibody
Application:
IHC, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$288
LS-C783060-25
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CASP8 / Caspase 8 Mouse anti-Human Monoclonal Antibody
Application:
IHC, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 8 antibody LS-C783060 is an unconjugated mouse monoclonal antibody to Caspase 8 (CASP8) from human. It is reactive with human and mouse. Validated for Flow, IHC and WB.
Target
Human CASP8 / Caspase 8
Synonyms
CASP8 | ALPS2B | Apoptotic protease Mch-5 | Apoptotic cysteine protease | Casp-8 | Caspase-8 | CAP4 | Caspase 8 | MACH | MCH5 | MORT1-associated ced-3 homolog | ICE-like apoptotic protease 5 | Pro Caspase 8 | FADD-like ICE | FLICE | MACH-alpha-1/2/3 protein | MACH-beta-1/2/3/4 protein
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
A partial length recombinant protein (aa 179-385) of Caspase-8
Applications
  • IHC (5 - 10 µg/ml)
  • Western blot (4 - 6 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS with 0.05% BSA and 0.05% sodium azide.
Storage
Store at 4°C for up to 6 months. For long-term storage, store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP8 / Caspase 8
Q14790 nm_001228 AAC50645.1

Publications (0)

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See all CASP8 / Caspase 8 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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