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Catalog Number Size Price
LS-C808530-100 100 µl (1 mg/ml) $379 
LS-C808530-200 200 µl (1 mg/ml) $421 
CASP9 / Caspase 9 Antibody - 1:100 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CASP9 / Caspase 9 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CASP9 / Caspase 9 Antibody - Western blot analysis of Caspase 9 phosphorylation expression in TNF treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CASP9 / Caspase 9 Antibody - Western blot analysis of Phospho-Caspase 9 (Thr125) expression in various lysates
CASP9 / Caspase 9 Antibody - 1:100 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CASP9 / Caspase 9 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CASP9 / Caspase 9 Antibody - Western blot analysis of Caspase 9 phosphorylation expression in TNF treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CASP9 / Caspase 9 Antibody - Western blot analysis of Phospho-Caspase 9 (Thr125) expression in various lysates
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Polyclonal Rabbit anti‑Human CASP9 / Caspase 9 Antibody (phospho‑Thr125, IHC, IF, WB) LS‑C808530

Polyclonal Rabbit anti‑Human CASP9 / Caspase 9 Antibody (phospho‑Thr125, IHC, IF, WB) LS‑C808530

Antibody:
CASP9 / Caspase 9 Rabbit anti-Human Polyclonal (pThr125) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C808530-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CASP9 / Caspase 9 Rabbit anti-Human Polyclonal (pThr125) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 9 antibody LS-C808530 is an unconjugated rabbit polyclonal antibody to Caspase 9 (CASP9) (pThr125) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human CASP9 / Caspase 9
Synonyms
CASP9 | APAF3 | CASPASE-9c | ICE-LAP6 | MCH6 | ICE-like apoptotic protease 6 | APAF-3 | Apoptotic protease Mch-6 | CASP-9 | Caspase 9 | Caspase-9 | PPP1R56
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Caspase 9 around the phosphorylation site of Threonine 125.
Epitope
pThr125
Specificity
Phospho-Caspase 9 (Thr125) Antibody detects endogenous levels of Caspase 9 only when phosphorylated at Threonine 125.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP9 / Caspase 9
P55211 NM_001229 NP_001220.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/24/2024