Human CCL2 / MCP1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Immunoaffinity purified

Unmodified

E.coli derived recombinant Human MCP-1/MCAF.

Human MCP-1/MCAF

• Western blot (0.2 - 0.4 µg/ml)
• ELISA (1 - 2 µg/ml)

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 300 pg/ml of recombinant human MCP-1 when used with biotinylated antigen affinity purified anti-human MCP-1 (LS-C104657) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1-2 ng/lane, under reducing or non-reducing conditions.

Lyophilized from PBS

Reconstitute with sterile water

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13500 NCBI: NM_002982 NP_002973.1