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Catalog Number	Size	Price
LS-C154130-100	100 µg (1 mg/ml)	$567

CCL3 / MIP-1-Alpha Antibody - Anti-Mouse MIP-1a Antibody - Western Blot. Anti-mouse MIP-1a in western blot shows detection of recombinant mouse MIP-1a/Ccl3 raised in E. coli. Recombinant truncated (0.1 ug, 7.8 kD) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.

Polyclonal Rabbit anti‑Mouse CCL3 / MIP‑1‑Alpha Antibody (WB) LS‑C154130

Name: Anti-CCL3 / MIP-1-Alpha Antibody | Rabbit anti-Mouse Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C154130-100
Price: 567.0000 USD
Availability: InStock

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Antibody:

CCL3 / MIP-1-Alpha Rabbit anti-Mouse Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$567

LS-C154130-100

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

CCL3 / MIP-1-Alpha Rabbit anti-Mouse Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

MIP-1-Alpha antibody LS-C154130 is an unconjugated rabbit polyclonal antibody to mouse MIP-1-Alpha (CCL3). Validated for ELISA and WB.

Target

Mouse CCL3 / MIP-1-Alpha

Synonyms

See All CCL3 / MIP-1-Alpha Antibodies

Host

Rabbit

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Modifications

Unmodified

Immunogen

This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse MIP-1a protein produced in E.coli.

Specificity

This antibody is specific for mouse MIP-1a protein. Cross-reactivity with MIP-1a from other sources has not been determined.

Applications

Western blot (1:1000)
ELISA (1:10000)

Usage

This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7-8 kD in size corresponding to the mature 92 amino acid mouse MIP-1a/Ccl3 protein by western blotting in appropriate cell lysate or extract.

Presentation

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl

Reconstitution

Reconstitute in 100 µL of deionized water.

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About CCL3 / MIP-1-Alpha