Mouse CCR7

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

4B12

Unconjugated

Protein G purified

Unmodified

RBC-2H3 cells expressing mCCR7.

Recognizes mouse CCR7.

• IHC (25 µg/ml)
• Flow Cytometry
• Functional Assay
• Neutralization

Flow cytometry, Immunohistochemistry, Neutralization of Bioactivity. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: 25 ug/mL with appropriate secondary reagents to detect CCR7 on paraformaldehyde-fixed mouse splenocytes. Neutralization of Murine CCR7 Bioactivity: The exact concentration of antibody required to neutralize mouse CCR7 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-mouse CCR7 antibody was determined to be approximately 2-10 ug/mL in the presence of 25 ng/mL of rmMIP-3, using the BaF/3 mCCR7 cell line in a chemoattraction assay.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute in sterile PBS.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P32248 NCBI: NM_001838 NP_001829.1