Target
Mouse CD40
Synonyms
CD40 | B-cell surface antigen CD40 | B cell-associated molecule | B cell surface antigen CD40 | CD40 antigen | CD40 type II isoform | p50 | Bp50 | CD40L receptor | CDW40 | TNFRSF5
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
R. Phycoerythrin.
Also available
Unconjugated.
Purification
Protein G purified
Immunogen
Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1
Epitope
Extracellular Domain
Specificity
Recognizes the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity ofwas demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40. Is a powerful activator of normal B cells especially in the presence of IL-4.
Applications
- Flow Cytometry (1:1)
- Applications tested for the base form of this product only
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee