Human CD95 / FAS

Mouse

Human (tested or 100% immunogen sequence identity)

IgM Monoclonal

CH11

Unconjugated

Immunoaffinity purified

Unmodified

Human diploid fibroblast cell line FS-7.

Recognizes the human cell surface antigen Fas (~43kD) expressed in various human cells, including myeloid cells, T lymphoblastoid cells and diploid fibroblasts. This antibody does not recognize TNF and does not cross-react with mouse Fas.

• ICC
• Western blot
• Flow Cytometry

Suitable for use in Flow Cytometry, Western Blot, and Immunocytochemistry. Apoptosis: >15 ng/ml maximally induced apoptosis of human Jurkat cells with 80-90% mortality after 24 hours of treatment. This lot has an ID50 of 1.0 ng/ml in apoptosis assays. Western Blot: 0.5-2 mg/ml. Immunocytochemistry: 5-10 mg/ml o. Flow cytometry: Use 20 mg/ml. Can be used for activation studies.

PBS, pH 7.2, 50% Glycerol

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P25445 NCBI: NM_152877 NP_000034.1