Target
Mouse CD95 / FAS
Synonyms
FAS | ALPS1A | APO-1 | APT1 | Apo-1 antigen | APO-1 cell surface antigen | Apo11 antigen | Apoptosis antigen 1 | CD95 antigen | Delta Fas/APO-1/CD95 | Fas AMA | FAS1 | Fasl receptors | FASLG receptor | FASTM | FAS 827dupA | Fasl receptor | TNFRSF6 | CD95
Reactivity
Mouse, Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Recombinant protein containing the cytoplasmic domain of mouse Fas.
Epitope
Cytoplasmic Domain
Specificity
Recognizes mouse Fas at about 45kD. Species cross-reactivity: bovine.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects Fas (~45 kD) in RIPA lysates of mouse 3T3/A31 fibroblast cells and bovine brain. Immunoprecipitation: 4 ug immunoprecipitates Fas from 3T3 cells. RIP, a death domain containing protein, was co-precipitated with Fas.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee