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Catalog Number	Size	Price
LS-C407773-10	10 µg	$318
LS-C407773-100	100 µg	$470

CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in frozen section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CDH1 / E Cadherin Antibody - E Cadherin antibody IHC-paraffin. IHC(P): Human Placenta Tissue.

CDH1 / E Cadherin Antibody - E Cadherin antibody IHC-frozen. IHC(F): Human Placenta Tissue.

CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CDH1 / E Cadherin Antibody - E Cadherin antibody Western blot. All lanes: Anti E Cadherin at 0.5 ug/ml. Lane 1: Human Placenta Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Predicted band size: 140 kD. Observed band size: 140 kD.

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Polyclonal Rabbit anti‑Human CDH1 / E Cadherin Antibody (aa286‑703, IHC, WB) LS‑C407773

Name: Anti-CDH1 / E Cadherin Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407773-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

CDH1 / E Cadherin Rabbit anti-Human Polyclonal (aa286-703) Antibody

Application:

IHC, IHC-P, IHC-Fr, WB, ELISA

Reactivity:

Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep

Format:

Unconjugated, Unmodified

$318

LS-C407773-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CDH1 / E Cadherin Rabbit anti-Human Polyclonal (aa286-703) Antibody

Application:

IHC, IHC-P, IHC-Fr, WB, ELISA

Reactivity:

Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep

Format:

Unconjugated, Unmodified

Specifications

Description

E Cadherin antibody LS-C407773 is an unconjugated rabbit polyclonal antibody to E Cadherin (CDH1) (aa286-703) from human. It is reactive with human, rat, bat and other species. Validated for ELISA, IHC and WB.

Target

Human CDH1 / E Cadherin

Synonyms

See All CDH1 / E Cadherin Antibodies

Host

Rabbit

Reactivity

Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.

Epitope

aa286-703

Specificity

Non-neural epithelial tissues.

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
IHC - Frozen (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
ELISA (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About CDH1 / E Cadherin

Uniprot: P12830 NCBI: NM_004360 NP_004351.1