Target
Human CMA1 / Mast Cell Chymase
Synonyms
CMA1 | Chymase | Chymase, heart | Chymase, mast cell | CYH | Mast cell chymase | Mcpt5 | MCT1 | Mast cell protease 5 | Mast cell protease I | Heart chymase | Alpha-chymase | Chymase 1 | Chymase 1, mast cell | CYM
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
NS0-derived recombinant human Chymase (aa20-247) Accession # P23946.
Specificity
Recognizes human Chymase. Cross-reactivity: Approximately 10% with rmMCPT1 and <1% with rmMCPT6.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Immunoprecipitation
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Western Blot, Immunoprecipitation and ELISA. Western Blot: 0.1-0.2 ug/ml. Detection limit is ~5 ng/lane under non-reducing or reducing conditions. ELISA: 0.5-1 ug/ml. Detection limit is ~1.5 ng/well. Immunoprecipitation: Immunoprecipitate rhChymase. from conditioned media of transfected NS0 cells.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute with 500uL of sterile PBS.
Storage
Lyophilized powder may be stored at -20°C for short-term only. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CMA1 / Mast Cell Chymase