Target
Human CMA1 / Mast Cell Chymase
Synonyms
CMA1 | Chymase | Chymase, heart | Chymase, mast cell | CYH | Mast cell chymase | Mcpt5 | MCT1 | Mast cell protease 5 | Mast cell protease I | Heart chymase | Alpha-chymase | Chymase 1 | Chymase 1, mast cell | CYM
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rhChymase. (aa 20 - 247).
Specificity
Recognizes human Chymase at ~30kD. No cross reactivity with rmMCPT-1, rmMCPT-6, rmMCPT-7 or rmMCPT-11.
Applications
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1 ug/ml. Detection limit for rhChymase is ~1 ng/well. Western Blot: 1-2 ug/ml. Detection limit for rhChymase is. ~10 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 ug/ml from conditioned cell culture medium.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CMA1 / Mast Cell Chymase