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Catalog Number Size Price
LS-C827904-0.1 0.1 mg (1 mg/ml) $482 
CYBB / NOX2 / gp91phox Antibody

Monoclonal Mouse anti‑Human CYBB / NOX2 / gp91phox Antibody (clone 54.1, IHC, IF, WB) LS‑C827904

Monoclonal Mouse anti‑Human CYBB / NOX2 / gp91phox Antibody (clone 54.1, IHC, IF, WB) LS‑C827904

Antibody:
CYBB / NOX2 / gp91phox Mouse anti-Human Monoclonal (54.1) Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$482
LS-C827904-0.1
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CYBB / NOX2 / gp91phox Mouse anti-Human Monoclonal (54.1) Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Gp91phox antibody LS-C827904 is an unconjugated mouse monoclonal antibody to human gp91phox (CYBB / NOX2). Validated for ICC, IF, IHC and WB.
Target
Human CYBB / NOX2 / gp91phox
Synonyms
CYBB | Chronic granulomatous disease | CGD91-phox | Cytochrome b558 subunit beta | Cytochrome b(558) subunit beta | gp91-1 | gp91-PHOX | p22 phagocyte B-cytochrome | AMCBX2 | CGD | Cytochrome b-245 heavy chain | gp91PHOX | NADPH oxidase 2 | NOX2 | p91-PHOX
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
54.1
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)
Specificity
This antibody is specific for NOX2/gp91phox 382-PKIAVDGP-389
Applications
  • IHC - Paraffin (5 µg/ml)
  • ICC (1 µg/ml)
  • Immunofluorescence (1 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Optimal antibody concentration should be determined by titration. However as a guideline try; WB: use at an assay dependent dilution. Detects a band of approximately 65 kDa. Predicted molecular weight: 65 kDa. ICC/IF: Use a concentration of 1 µg/ml. IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Presentation
PBS, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYBB / NOX2 / gp91phox
P04839 NM_000397 NP_000388.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/22/2024