Monoclonal Mouse anti‑Human CYBB / NOX2 / gp91phox Antibody (clone 54.1, IHC, IF, WB) LS‑C827904
Monoclonal Mouse anti‑Human CYBB / NOX2 / gp91phox Antibody (clone 54.1, IHC, IF, WB) LS‑C827904
Antibody:
CYBB / NOX2 / gp91phox Mouse anti-Human Monoclonal (54.1) Antibody
Application:
IHC-P, ICC, IF, WB
Format:
Unconjugated, Unmodified
Overview
Antibody:
CYBB / NOX2 / gp91phox Mouse anti-Human Monoclonal (54.1) Antibody
Application:
IHC-P, ICC, IF, WB
Format:
Unconjugated, Unmodified
Specifications
Description
Gp91phox antibody LS-C827904 is an unconjugated mouse monoclonal antibody to human gp91phox (CYBB / NOX2). Validated for ICC, IF, IHC and WB.
Target
Human CYBB / NOX2 / gp91phox
Synonyms
CYBB | Chronic granulomatous disease | CGD91-phox | Cytochrome b558 subunit beta | Cytochrome b(558) subunit beta | gp91-1 | gp91-PHOX | p22 phagocyte B-cytochrome | AMCBX2 | CGD | Cytochrome b-245 heavy chain | gp91PHOX | NADPH oxidase 2 | NOX2 | p91-PHOX
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Immunogen
Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)
Specificity
This antibody is specific for NOX2/gp91phox 382-PKIAVDGP-389
Applications
- IHC - Paraffin (5 µg/ml)
- ICC (1 µg/ml)
- Immunofluorescence (1 µg/ml)
- Western blot
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Optimal antibody concentration should be determined by titration. However as a guideline try; WB: use at an assay dependent dilution. Detects a band of approximately 65 kDa. Predicted molecular weight: 65 kDa. ICC/IF: Use a concentration of 1 µg/ml. IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Presentation
PBS, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYBB / NOX2 / gp91phox
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024