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Catalog Number Size Price
LS-C815588-100 100 µl $412 
ER Alpha / Estrogen Receptor Antibody - Immunofluorescence staining of ER alpha/ESR1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ER alpha/ESR1 polyclonal antibody (dilution ratio: 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
ER Alpha / Estrogen Receptor Antibody - Anti-ER alpha/ESR1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Molt4 Whole Cell Lysate. Lane B: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 67 kDa. Observed band size: 67 kDa. (We are unsure as to the identity of these extra bands.)
ER Alpha / Estrogen Receptor Antibody - Immunofluorescence staining of ER alpha/ESR1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ER alpha/ESR1 polyclonal antibody (dilution ratio: 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
ER Alpha / Estrogen Receptor Antibody - Anti-ER alpha/ESR1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Molt4 Whole Cell Lysate. Lane B: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 67 kDa. Observed band size: 67 kDa. (We are unsure as to the identity of these extra bands.)
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Polyclonal Rabbit anti‑Human ER Alpha / Estrogen Receptor Antibody (Preservative Free, IF, WB) LS‑C815588

Polyclonal Rabbit anti‑Human ER Alpha / Estrogen Receptor Antibody (Preservative Free, IF, WB) LS‑C815588

Antibody:
ER Alpha / Estrogen Receptor Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C815588-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ER Alpha / Estrogen Receptor Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
Estrogen Receptor antibody LS-C815588 is an unconjugated rabbit polyclonal antibody to human Estrogen Receptor (ER Alpha). Validated for ICC, IF and WB.
Target
Human ER Alpha / Estrogen Receptor
Synonyms
ESR1 | Era | ERalpha | Estrogen receptor | ER-alpha | Estrogen receptor 1 | NR3A1 | ER | ESR | ESRA | Estrogen receptor alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
A synthetic peptide corresponding to the N-Terminus of Human ER alpha/ESR1
Specificity
Human ER alpha
Applications
  • ICC (1:200 - 1:000)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ER Alpha / Estrogen Receptor
P03372 NM_000125 NP_000116.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/22/2024