Human F2R / Thrombin Receptor / PAR1

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

E. coli-derived recombinant human PAR1 (aa 27-102; 375-428).

aa27-102

Selected for its ability to recognize human PAR1 in the applications listed.

• Western blot
• Flow Cytometry
• ELISA

Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody was tested in flow cytometry using whole blood granulocytes. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human PAR1. The detection limit for rhPAR1 is approximately 0.2 ng/well.

Lyophilized

Reconstitute in deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P25116 NCBI: NM_001992 NP_001983.2