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Antibodies > F8 / FVIII / Factor VIII > LS-C800279
 
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Catalog Number Size Price
LS-C800279-100 100 µl (1 mg/ml) $379 
LS-C800279-200 200 µl (1 mg/ml) $421 
F8 / FVIII / Factor VIII Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
F8 / FVIII / Factor VIII Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
F8 / FVIII / Factor VIII Antibody - Western blot analysis of extracts of HeLa cells using Factor VIII antibody.
F8 / FVIII / Factor VIII Antibody - Western blot analysis on HuvEc cell lysates using Factor VIII antibody. The lane on the left is treated with the antigen-specific peptide.
F8 / FVIII / Factor VIII Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
F8 / FVIII / Factor VIII Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
F8 / FVIII / Factor VIII Antibody - Western blot analysis of extracts of HeLa cells using Factor VIII antibody.
F8 / FVIII / Factor VIII Antibody - Western blot analysis on HuvEc cell lysates using Factor VIII antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human F8 / FVIII / Factor VIII Antibody (aa2187‑2209, IHC, IF, WB) LS‑C800279

Polyclonal Rabbit anti‑Human F8 / FVIII / Factor VIII Antibody (aa2187‑2209, IHC, IF, WB) LS‑C800279

Submit Review LSBio Guarantee
Antibody:
F8 / FVIII / Factor VIII Rabbit anti-Human Polyclonal (aa2187-2209) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800279-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
F8 / FVIII / Factor VIII Rabbit anti-Human Polyclonal (aa2187-2209) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Factor VIII antibody LS-C800279 is an unconjugated rabbit polyclonal antibody to Factor VIII (F8 / FVIII) (aa2187-2209) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human F8 / FVIII / Factor VIII
Synonyms
F8 | AHF | Antihemophilic factor | Coagulation factor VIIIc | Coagulation factor VIII | DXS1253E | Factor VIII F8B | FVIII | HEMA | F8B | Factor VIII | Procoagulant component | F8C
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthesized peptide derived from human Factor VIII
Epitope
aa2187-2209
Specificity
Factor VIII antibody detects endogenous levels of total Factor VIII.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About F8 / FVIII / Factor VIII
P00451 NM_000132 NP_000123.1

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Featured Products

F8 / FVIII / Factor VIII Antibody - Western blot of. Factor VIII (S2194) pAb in extracts from HUVEC cells.
F8 / FVIII / Factor VIII Antibody (Ser2194) LS‑C176016
Species: Human, Mouse
Applications: Western blot
F8 / FVIII / Factor VIII Antibody - Anti-F8 / Factor VIII antibody IHC of human skeletal muscle and capillaries. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ F8 / FVIII / Factor VIII Antibody LS‑B2979
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
F8 / FVIII / Factor VIII Antibody - Anti-F8 / Factor VIII antibody IHC of human uterus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PathPlus™ F8 / FVIII / Factor VIII Antibody (clone F8 2.2.9) LS‑B3124
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
F8 / FVIII / Factor VIII Antibody - F8 / Factor VIII antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
F8 / FVIII / Factor VIII Antibody (aa1790‑1839) LS‑C135701
Species: Human
Applications: Western blot
Product
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Species: Human
Applications: ELISA, Immunoelectrophoresis
F8 / FVIII / Factor VIII Antibody - Western Blot; Sample: Recombinant protein.
F8 / FVIII / Factor VIII Antibody (aa1669‑1824, Biotin) LS‑C300526
Species: Pig
Applications: Western blot
See all F8 / FVIII / Factor VIII Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/25/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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