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Catalog Number	Size	Price
LS-C408077-10	10 µg	$318
LS-C408077-100	100 µg	$470

FASN / Fatty Acid Synthase Antibody - IHC analysis of FASN using anti-FASN antibody. FASN was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

FASN / Fatty Acid Synthase Antibody - IHC analysis of FASN using anti-FASN antibody. FASN was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

FASN / Fatty Acid Synthase Antibody - FASN was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- FASN Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.

FASN / Fatty Acid Synthase Antibody - Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273KD was detected using rabbit anti-FASN Antigen Affinity purified polyclonal antibody at0.5 ug/ml. The blot was developed using chemiluminescence (ECL) method.

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Polyclonal Rabbit anti‑Human FASN / Fatty Acid Synthase Antibody (aa1‑226, IHC, WB) LS‑C408077

Name: Anti-FASN / Fatty Acid Synthase Antibody | Rabbit anti-Human IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C408077-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

FASN / Fatty Acid Synthase Rabbit anti-Human Polyclonal (aa1-226) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C408077-10

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

FASN / Fatty Acid Synthase Rabbit anti-Human Polyclonal (aa1-226) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Fatty Acid Synthase antibody LS-C408077 is an unconjugated rabbit polyclonal antibody to human Fatty Acid Synthase (FASN) (aa1-226). Validated for IHC and WB.

Target

Human FASN / Fatty Acid Synthase

Synonyms

FASN | Fatty acid synthase | OA-519 | SDR27X1

See All FASN / Fatty Acid Synthase Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.

Epitope

aa1-226

Specificity

Ubiquitous. Prominent expression in brain, lung, and liver. .

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About FASN / Fatty Acid Synthase

Uniprot: P49327 NCBI: NM_004104 NP_004095.4