Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
FITC.
Also available
Unconjugated or conjugated with
Biotin,
HRP.
Purification
Purified IgG
Immunogen
Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Mouse fibrinogen.
Applications
- IHC
- IHC - Frozen
- ICC
- Immunofluorescence
- ELISA
- Immunodiffusion
- Applications tested for the base form of this product only
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Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As reagent for the direct detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence. as detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee