Human GDF15

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

Chimpanzee, Macaque (at least 90% immunogen sequence identity)

IgG Polyclonal

Biotin

Affinity purified

Unmodified

Nag-1 Antibody Biotin Conjugated antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.

This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.

• IHC (1:1000 - 1:5000)
• Western blot (1:2000 - 1:10000)
• ELISA (1:20000 - 1:100000)

Nag-1 Antibody Biotin Conjugated antibody is suitable for ELISA and western blotting of human and mouse NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of approximately 14 and 28 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Store at 4°C prior to resoration. Aliquot and store at -20°C for 12 months. Avoid freeze-thaw cycles. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99988 NCBI: NM_004864 NP_004855.2