Mouse GDF15

Rabbit

Mouse, Human (tested or 100% immunogen sequence identity)

Bovine, Dog, Pig (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared by repeated immunizations with a peptide corresponding to an amino acid sequence near the C-terminal of mouse NAG-1 protein.

C-Terminus

This antibody reacts with endogenous NAG-1 protein from mouse and human tissues. A BLAST analysis suggests reactivity with NAG-1 from rat based on 100% homology. Partial reactivity is expected against swine, bovine and dog based on 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.

• Western blot (1:500 - 1:2000)
• ELISA (1:100000 - 1:120000)

This affinity purified antibody is suitable for ELISA and western blotting of mouse and human NAG-1 protein. For detection of NAG-1 in mouse serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal) specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of approximately 14 and 28 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99988 NCBI: NM_004864 NP_004855.2