Human GDF15

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b,k Monoclonal

7C12.B3.F2

Unconjugated

Protein A affinity chromatography

Unmodified

This Protein A purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.

This antibody specifically reacts with an H variant sequence of human NAG-1 protein from human tissues.  A BLAST analysis was used to suggest partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.

• Western blot (1:1000)
• ELISA (1:150000)

This Protein A purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. This reagent is particularly useful to differentiate polymorphic forms of NAG-1 protein present in human serum samples. This antibody is useful in dual antibody immunometric assays (EIA). Expect bands in Western blots of approximately 14 and 28 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99988 NCBI: NM_004864 NP_004855.2