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Antibodies > GDF15 > LS-C154239
 
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Catalog Number Size Price
LS-C154239-100 100 µg (1 mg/ml) $567 
GDF15 Antibody - Anti-NAG-1 (H variant specific) Antibody - ELISA. In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies (see Related Products below): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 ug/mL. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 ul per well at 1 ug/mL. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.

Polyclonal Rabbit anti‑Human GDF15 Antibody (IHC, WB) LS‑C154239

Polyclonal Rabbit anti‑Human GDF15 Antibody (IHC, WB) LS‑C154239

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Antibody:
GDF15 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C154239-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GDF15 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GDF15 antibody LS-C154239 is an unconjugated rabbit polyclonal antibody to human GDF15. Validated for ELISA, IHC and WB.
Target
Human GDF15
Synonyms
GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.
Specificity
This antibody specifically reacts with an H variant sequence of human NAG-1 protein from human tissues.  A BLAST analysis was used to suggest partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Cross-reactivity with NAG-1 from other sources has not been determined.
Applications
  • IHC
  • Western blot (1:1000 - 1:2000)
  • ELISA (1:2500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody is suitable for use in ELISA and western blotting assays. This reagent is particularly useful to differentiate polymorphic forms of NAG-1 protein present in human serum samples. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GDF15
Q99988 NM_004864 NP_004855.2

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GDF15 Antibody - Anti-NAG-1 (D variant specific) Antibody - ELISA. In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies (see Related Products below): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 ug/mL. Control plates received PBS only (data not shown). After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. After washing, HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 ul per well at 1 ug/mL. Following further incubation for 1 hr at 37°C, the plates were washed and TMBE was added as an HRP substrate for 30 min. The reaction was stopped by 1 M H2SO4 and values were measured at 450nm.
GDF15 Antibody LS‑C154240
Species: Human
Applications: IHC, Western blot, ELISA
GDF15 Antibody - Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
GDF15 Antibody (C‑Terminus, HRP) LS‑C154280
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
See all GDF15 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
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