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Catalog Number	Size	Price
LS-C353529-100	100 µl (1 mg/ml)	$299

IFIH1 / MDA5 Antibody - Immunohistochemical analysis of MDA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

IFIH1 / MDA5 Antibody - Immunofluorescent analysis of MDA5 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IFIH1 / MDA5 Antibody - Western blot analysis of MDA5 expression in HepG2 (A); THP1 (B) whole cell lysates.

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Polyclonal Rabbit anti‑Human IFIH1 / MDA5 Antibody (C‑Terminus, IHC, IF, WB) LS‑C353529

Name: Anti-IFIH1 / MDA5 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C353529-100
Price: 299.0000 USD
Availability: InStock

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Antibody:

IFIH1 / MDA5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

$299

LS-C353529-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IFIH1 / MDA5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

MDA5 antibody LS-C353529 is an unconjugated rabbit polyclonal antibody to MDA5 (IFIH1) (C-Terminus) from human. It is reactive with human and rat. Validated for ICC, IF, IHC and WB.

Target

Human IFIH1 / MDA5

Synonyms

See All IFIH1 / MDA5 Antibodies

Host

Rabbit

Reactivity

Human, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human MDA5.

Epitope

C-Terminus

Specificity

Recognizes endogenous levels of MDA5 protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IFIH1 / MDA5

Uniprot: Q9BYX4 NCBI: NM_022168 NP_071451.2