Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (800) 227-6666


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order

IFIH1 / MDA5

interferon induced with helicase C domain 1

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.

Gene Name: interferon induced with helicase C domain 1
Family/Subfamily: DEAD box protein
Synonyms: IFIH1, Helicase with 2 CARD domains, MDA-5, MDA5, Helicard, IDDM19, RLR-2, RH116, CADM-140 autoantigen, Hlcd, RIG-I-like receptor 2
Target Sequences: NM_022168 NP_071451.2 Q9BYX4

Publications (1)

1
RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease. Sato S, Hoshino K, Satoh T, Fujita T, Kawakami Y, Fujita T, Kuwana M. Arthritis and rheumatism. 2009 60:2193-200. [PubMed:19565506] Related Antibodies: LS-C55097.

☰ Filters
Antibodies (55)
Proteins (8)
ELISA Kits (4)
Over-Expression Lysate (2)
Primary (55)
Recombinant (6)
Sandwich (4)
IFIH1 / MDA5 (67)
Human (56)
Mouse (15)
Rat (4)
Bovine (3)
Chimpanzee (3)
Horse (3)
Monkey (1)
Pig (3)
IHC (13)
IHC-P (12)
WB (46)
ELISA (14)
ICC (1)
IF (6)
IP (5)
Peptide-ELISA (4)
rabbit (38)
mouse (14)
goat (3)
Product Group
IHCPlus (3)
PathPlus Cancer (1)
IgG (24)
IgG1 (8)
IgG2a (4)
IgG2a,k (1)
monoclonal mc (14)
polyclonal pc (41)
1B6 (1)
3F6 (1)
Hely-1 (1)
OTI11C11 (2)
OTI12A6 (2)
OTI1C7 (2)
OTI3E10 (2)
OTI8C10 (2)
96-Well Strip Plate (4)
APC (1)
APC Conjugated (2)
Biotin Conjugated (4)
Carrier-free (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (2)
Unconjugated (37)
aa605-815 (8)
aa612-661 (4)
940-1020 aa, C-terminal (1)
Arg470 (1)
C-Terminus (1)
Internal Region (1)
N-Terminal (1)
N-Terminus (1)
aa1-205 (1)
aa78-555 (1)
aa976-1025 (1)
No (66)
Yes (1)
Cell Culture Supernatants (1)
Cell Lysates (1)
Plasma (3)
Serum (3)
Tissue Homogenates (3)
6His, N-terminus (1)
6His-SUMO, N-terminus (1)
GST (1)
GST, N-terminus (1)
His (1)
Myc-DDK (Flag) (3)
Human (7)
293T Cells (1)
E. coli (3)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (3)
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$545
Select
IFIH1 / MDA5 Rabbit anti-Human Monoclonal (Arg470) Antibody
Mouse, Human
IP, WB
Unconjugated
100 µl/$688
IFIH1 / MDA5 Antibody - Immunohistochemical analysis of MDA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$299
IFIH1 / MDA5 Antibody - Immunoperoxidase of monoclonal antibody to IFIH1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Select
IFIH1 / MDA5 Mouse anti-Human Monoclonal (3F6) Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
100 µg/$423
IFIH1 / MDA5 Antibody - Antibody (10 ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Select
IFIH1 / MDA5 Goat anti-Human Polyclonal (N-Terminus) Antibody
Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
100 µg/$503
IFIH1 / MDA5 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Mouse
31.2 - 2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates
1 Plate/$669
IFIH1 / MDA5 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
78.125 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$587
IFIH1 / MDA5 Protein - Purified recombinant protein IFIH1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
116.5 kDa
20 µg/$1,107
IFIH1 / MDA5 Antibody - Immunohistochemistry of paraffin-embedded human colon cancer tissue.
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Human
IF, IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
IFIH1 / MDA5 Antibody - Western blot.
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
IFIH1 / MDA5 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Select
Cancer
Fast Shipping
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
IHC-P
Unconjugated
50 µg/$460
IFIH1 / MDA5 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
78 - 5000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$669
IFIH1 / MDA5 Antibody - Anti-MDA5 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal (aa612-661) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Human
IP, WB
Unconjugated
100 µg/$563
IFIH1 / MDA5 Antibody - Immunohistochemistry of paraffin-embedded human placenta tissue using IFIH1 Antibody at dilution of 1:100
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Human
ELISA
Unconjugated
50 µg/$294; 100 µg/$360
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
IFIH1 / MDA5 Antibody - Western blot of IFIH1 / MDA5 antibody.
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal (aa605-815) (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$329; 200 µl/$381; 500 µl/$550; 1 ml/$654; 2 ml/$890; 5 ml/$1,487
IFIH1 / MDA5 Protein - Recombinant Interferon Induced Helicase C Domain Containing Protein 1 By SDS-PAGE
Select
E. coli
His
28.2 kDa
10 µg/$279; 50 µg/$427; 100 µg/$682; 200 µg/$852; 1 mg/$1,950; 500 µg/$1,494; 5 mg/$2,983; 2 mg/$2,136
IFIH1 / MDA5 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Select
E. coli
6His, N-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
IFIH1 / MDA5 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
IFIH1 / MDA5 Antibody - Western blot of MDA5 antibody
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal (940-1020 aa, C-terminal) Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
IFIH1 / MDA5 Antibody - Immunohistochemistry of paraffin-embedded human pancreas using antibody at 1:100 dilution.
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal (aa1-205) Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µl/$294; 100 µl/$360
Select
IFIH1 / MDA5 Mouse anti-Human Monoclonal (aa78-555) (Hely-1) Antibody
Human
ELISA, IP, WB
Unconjugated
100 µg/$477
Select
IFIH1 / MDA5 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IP, WB
Unconjugated
100 µg/$351
Viewing 1-25 of 67 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).