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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16695-50 | 50 µg (0.5 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Goat anti‑Human BMI1 / PCGF4 Antibody (aa303‑315, IHC, WB) LS‑B16695
IHC‑plus™ Polyclonal Goat anti‑Human BMI1 / PCGF4 Antibody (aa303‑315, IHC, WB) LS‑B16695
Note: This antibody replaces LS-C305865
Antibody:
BMI1 / PCGF4 Goat anti-Human Polyclonal (aa303-315) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
BMI1 / PCGF4 Goat anti-Human Polyclonal (aa303-315) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PCGF4 antibody LS-B16695 is an unconjugated goat polyclonal antibody to human PCGF4 (BMI1) (aa303-315). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BMI1 / PCGF4
Synonyms
BMI1 | BMI-1 | Flvi-2/bmi-1 | FLVI2/BMI1 | PCGF4 | Polycomb group protein Bmi1 | Polycomb complex protein BMI-1 | Dna-binding protein bmi-1 | Polycomb group ring finger 4 | RING finger protein 51 | RNF51
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-NHQSSFANRPRKS, from the C-Terminus of protein sequence according to NP_005171.4.
Epitope
aa303-315
Specificity
Human BMI1 / PCGF4
Applications
- IHC - Paraffin (5 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 40kD band observed in nuclear lysates of cell line K562 (calculated MW of 36.9kD according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev. 2006 Aug 1.20(15):2110-20. PMID: 16882984). Recommended concentration: 0.03-0.1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About BMI1 / PCGF4
Validation
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ BMI1 / PCGF4 Antibody (aa303-315) for IHC, WB/Western LS-B16695 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rabbit
Applications:
IHC, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
ICC, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/24/2024
Date Requested: 11/24/2024
