Target
Mouse CASP6 / Caspase 6
Synonyms
CASP6 | Apoptotic protease Mch-2 | Caspase 6 | CASP-6 | Caspase-6 | Mch-2 | MCH2
Reactivity
Mouse, Human, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic peptide KLH coupled corresponding to residues surrounding the cleavage site of caspase-6.
Specificity
Caspase-6 Antibody recognizes endogenous levels of both full length caspase-6 (35kD) and the small subunit of caspase-6 resulting from cleavage at Asp194 (15kD). This antibody does not recognize other caspases. Recognizes human, mouse, rabbit.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000)
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2679 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2679 was determined to be 5 ug/ml.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee