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Catalog Number Size Price
LS-B2020-50 50 µg (0.5 mg/ml) $460 
CASP7 / Caspase 7 Antibody - Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CASP7 / Caspase 7 Antibody - Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.
CASP7 / Caspase 7 Antibody - Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.
CASP7 / Caspase 7 Antibody - Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CASP7 / Caspase 7 Antibody - Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.
CASP7 / Caspase 7 Antibody - Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.
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IHC‑plus™ Monoclonal Mouse anti‑Human CASP7 / Caspase 7 Antibody (clone 25B881.1, IHC, WB) LS‑B2020

IHC‑plus™ Monoclonal Mouse anti‑Human CASP7 / Caspase 7 Antibody (clone 25B881.1, IHC, WB) LS‑B2020

Note: This antibody replaces LS-C506
Antibody:
CASP7 / Caspase 7 Mouse anti-Human Monoclonal (25B881.1) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B2020-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CASP7 / Caspase 7 Mouse anti-Human Monoclonal (25B881.1) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 7 antibody LS-B2020 is an unconjugated mouse monoclonal antibody to Caspase 7 (CASP7) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CASP7 / Caspase 7
Synonyms
CASP7 | Apoptotic protease Mch-3 | Caspase 7 | Caspase-7 | ICE-LAP3 | LICE2 | MCH3 | ICE-like apoptotic protease 3 | CASP-7 | CMH-1
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
25B881.1
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human Caspase-7.
Specificity
In Jurkat, a 35 kD band is observed.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2020 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2020 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP7 / Caspase 7
P55210 NM_001227 NP_001218.1

Validation

CASP7 / Caspase 7 Antibody - Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ CASP7 / Caspase 7 Antibody (clone 25B881.1) for IHC, WB/Western LS-B2020 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024