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| Catalog Number | Size | Price |
|---|---|---|
| LS-B240-50 | 50 µl | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human CASP8 / Caspase 8 Antibody (clone FLICE 4‑1‑20, IHC, WB) LS‑B240
IHC‑plus™ Monoclonal Mouse anti‑Human CASP8 / Caspase 8 Antibody (clone FLICE 4‑1‑20, IHC, WB) LS‑B240
Note: This antibody replaces LS-C2774
Antibody:
CASP8 / Caspase 8 Mouse anti-Human Monoclonal (FLICE 4-1-20) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CASP8 / Caspase 8 Mouse anti-Human Monoclonal (FLICE 4-1-20) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Caspase 8 antibody LS-B240 is an unconjugated mouse monoclonal antibody to human Caspase 8 (CASP8). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CASP8 / Caspase 8
Synonyms
CASP8 | ALPS2B | Apoptotic protease Mch-5 | Apoptotic cysteine protease | Casp-8 | Caspase-8 | CAP4 | Caspase 8 | MACH | MCH5 | MORT1-associated ced-3 homolog | ICE-like apoptotic protease 5 | Pro Caspase 8 | FADD-like ICE | FLICE | MACH-alpha-1/2/3 protein | MACH-beta-1/2/3/4 protein
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
FLICE 4-1-20
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Full-length recombinant human Caspase 8 protein.
Specificity
Specific for Caspase 8.
Applications
- IHC
- IHC - Paraffin (1:50)
- Western blot (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B240 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B240 was determined to be 1:50.
Presentation
0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CASP8 / Caspase 8
Validation
Anti-Caspase 8 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-Caspase 8 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
See More About...
LSBio Ratings
IHC-plus™ CASP8 / Caspase 8 Antibody (clone FLICE 4-1-20) for IHC, WB/Western LS-B240 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
