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Catalog Number Size Price
LS-B16842-50 50 µl (1 mg/ml) $460 
CASP9 / Caspase 9 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CASP9 / Caspase 9 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
CASP9 / Caspase 9 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CASP9 / Caspase 9 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CASP9 / Caspase 9 Antibody - Western blot analysis of Caspase 9 (Cleaved-Asp353) expression in NIH-3T3 cells treated with etoposide. The lane on the left is treated with the antigen-specific peptide.
CASP9 / Caspase 9 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
CASP9 / Caspase 9 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
CASP9 / Caspase 9 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
CASP9 / Caspase 9 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CASP9 / Caspase 9 Antibody - Western blot analysis of Caspase 9 (Cleaved-Asp353) expression in NIH-3T3 cells treated with etoposide. The lane on the left is treated with the antigen-specific peptide.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CASP9 / Caspase 9 Antibody (Cleaved Asp353, IHC, IF, WB) LS‑B16842

IHC‑plus™ Polyclonal Rabbit anti‑Human CASP9 / Caspase 9 Antibody (Cleaved Asp353, IHC, IF, WB) LS‑B16842

Note: This antibody replaces LS-C801355
Antibody:
CASP9 / Caspase 9 Rabbit anti-Human Polyclonal (Cleaved Asp353) Antibody
Application:
IHC-P, ICC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16842-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CASP9 / Caspase 9 Rabbit anti-Human Polyclonal (Cleaved Asp353) Antibody
Application:
IHC-P, ICC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 9 antibody LS-B16842 is an unconjugated rabbit polyclonal antibody to Caspase 9 (CASP9) (Cleaved Asp353) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CASP9 / Caspase 9
Synonyms
CASP9 | APAF3 | CASPASE-9c | ICE-LAP6 | MCH6 | ICE-like apoptotic protease 6 | APAF-3 | Apoptotic protease Mch-6 | CASP-9 | Caspase 9 | Caspase-9 | PPP1R56
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Caspase 9 (Cleaved-Asp353).
Epitope
Cleaved Asp353
Specificity
Caspase 9 (Cleaved-Asp353) Antibody detects endogenous levels of total Caspase 9 (Cleaved-Asp353).
Applications
  • IHC - Paraffin (10 µg/ml)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP9 / Caspase 9
P55211 NM_001229 NP_001220.2

Validation

CASP9 / Caspase 9 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

CASP9 / Caspase 9 Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

CASP9 / Caspase 9 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CASP9 / Caspase 9 Antibody (Cleaved Asp353) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B16842 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/24/2024