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Catalog Number Size Price
LS-B5327-0.05 0.05 mg (0.1 mg/ml) $440 
CD45 / LCA Antibody - Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD45 / LCA Antibody - Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD45 / LCA Antibody - Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CD45 / LCA Antibody - Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC‑plus™ Monoclonal Mouse anti‑Human CD45 / LCA Antibody (clone F10‑89‑4, FITC, IHC) LS‑B5327

IHC‑plus™ Monoclonal Mouse anti‑Human CD45 / LCA Antibody (clone F10‑89‑4, FITC, IHC) LS‑B5327

Note: This antibody replaces LS-C45410, LS-C45411
Antibody:
CD45 / LCA Mouse anti-Human Monoclonal (FITC) (F10-89-4) Antibody
Application:
IHC, IHC-P, Flo
Reactivity:
Human
Format:
FITC, Unmodified
Price
Catalog Number
$440
LS-B5327-0.05
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD45 / LCA Mouse anti-Human Monoclonal (FITC) (F10-89-4) Antibody
Application:
IHC, IHC-P, Flo
Reactivity:
Human
Format:
FITC, Unmodified

Specifications

Description
LCA antibody LS-B5327 is an FITC-conjugated mouse monoclonal antibody to human LCA (CD45). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human CD45 / LCA
Synonyms
PTPRC | B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
F10-89-4
Conjugations
FITC
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Human T lymphocytes
Specificity
Recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Clone Reacts with all forms of CD45 expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. This product is routinely tested in flow cytometry on human peripheral blood leucocytes.
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Flow Cytometry (1:1)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% Sodium Azide, 1% BSA
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD45 / LCA
P08575 NM_002838 NP_002829.3

Validation

CD45 / LCA Antibody - Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

CD45 / LCA Antibody - Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ CD45 / LCA Antibody (clone F10-89-4, FITC) for IHC, Flow LS-B5327 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/22/2024