Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers

Tel: +1 (800) 227-6666

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-B16725-50	50 µg	$460

CDH1 / E Cadherin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDH1 / E Cadherin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDH1 / E Cadherin Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

CDH1 / E Cadherin Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

CDH1 / E Cadherin Antibody - Immunofluorescence staining of HepG2 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CDH1 / E Cadherin Antibody - Western Blot Positive WB detected in: 293 whole cell lysate All Lanes: CDH1 antibody at 2.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 98, 91 KDa Observed band size: 125 KDa

CDH1 / E Cadherin Antibody - Immunoprecipitating CDH1 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CDH1 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CDH1 Antibody (6µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)

1 of 7

2 of 7

3 of 7

4 of 7

5 of 7

6 of 7

7 of 7

IHC‑plus™ Polyclonal Rabbit anti‑Human CDH1 / E Cadherin Antibody (IHC, IF, WB) LS‑B16725

Name: IHC-plus™ CDH1 / E Cadherin Polyclonal Antibody Rabbit | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16725-50
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

Submit Review LSBio Guarantee

Note: This antibody replaces LS-C680259

Antibody:

CDH1 / E Cadherin Rabbit anti-Human Polyclonal Antibody

Application:

IHC-P, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

Biotin HRP FITC

$460

LS-B16725-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CDH1 / E Cadherin Rabbit anti-Human Polyclonal Antibody

Application:

IHC-P, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

Biotin HRP FITC

Specifications

Description

E Cadherin antibody LS-B16725 is an unconjugated rabbit polyclonal antibody to human E Cadherin (CDH1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human CDH1 / E Cadherin

Synonyms

See All CDH1 / E Cadherin Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated. Also available conjugated with Biotin, HRP, FITC.

Purification

Protein G purified

Modifications

Unmodified

Immunogen

Recombinant Human Cadherin-1 protein (23-621AA)

Applications

IHC - Paraffin (10 µg/ml)
Immunofluorescence
Western blot
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CDH1 / E Cadherin

Uniprot: P12830 NCBI: NM_004360 NP_004351.1