Target
Human CYBB / NOX2 / gp91phox
Synonyms
CYBB | Chronic granulomatous disease | CGD91-phox | Cytochrome b558 subunit beta | Cytochrome b(558) subunit beta | gp91-1 | gp91-PHOX | p22 phagocyte B-cytochrome | AMCBX2 | CGD | Cytochrome b-245 heavy chain | gp91PHOX | NADPH oxidase 2 | NOX2 | p91-PHOX
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-SYLNFARKRIKNP, from the internal region of the protein sequence according to NP_000388.2.
Specificity
Human CYBB / gp91phox.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
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Usage
Immunohistochemistry: LS-B4137 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4137 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CYBB / NOX2 / gp91phox