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Catalog Number	Size	Price
LS-B648-50	50 µl (85 mg/ml)	$515

EGFR Antibody - Anti-EGFR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

EGFR Antibody - Western Blot - Anti-EGFR Antibody. Western blot of anti-EGFR antibody shows detection of a band at ~170 kD corresponding to human EGFR present in unstimulated (lane 1) and EGF (50 ng/ml for 15 min) stimulated (lane 2) A431 whole cell lysates (arrowhead). Approximately 30 ug of lysate was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).

EGFR Antibody - Immunoprecipitation/Western Blot - Anti-EGFR Antibody. Combined immunoprecipitation and Western blot of anti-EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15 at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 ul) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1. Other detection systems will yield similar results.

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IHC‑plus™ Polyclonal Rabbit anti‑Human EGFR Antibody (IHC, WB) LS‑B648

Name: IHC-plus™ EGFR Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B648-50
Price: 515.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C18794

Antibody:

EGFR Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$515

LS-B648-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

EGFR Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

EGFR antibody LS-B648 is an unconjugated rabbit polyclonal antibody to EGFR from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human EGFR

Synonyms

See All EGFR Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Antiserum

Modifications

Unmodified

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a peptide synthesized using conventional technology. The sequence of the epitope maps to a region near the carboxy terminus which is identical in human, mouse and rat EGFR.

Specificity

This antiserum is directed against human epidermal growth factor receptor (EGFR) and is useful in determining its presence in western blotting and immunoprecipitation experiments. This antibody can detect EGFR from human, mouse and rat sources. Reactivity of this antibody with EGFR from other species is unknown.

Applications

IHC
IHC - Paraffin (1:500)
Western blot (1:1000 - 1:10000)
Immunoprecipitation
ELISA (1:10000 - 1:50000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B648 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B648 was determined to be 1:500.

Presentation

Antiserum, 0.01% Sodium Azide

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About EGFR

Uniprot: P00533 NCBI: NM_005228 NP_005219.2

Validation

Anti-EGFR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

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