Human EMA / MUC1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG Monoclonal

Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

High molecular weight (>300kD) glycoprotein from human milk-fat globule molecule.

Recognizes underglycosylated and natural MUC1 protein (isolated from human milk by affinity chromatography). Does not cross-react with normal serum proteins and human tissues of nonepithelial origin. Binds with high efficiency with different epitopes within the VNTR tandem repeat peptide conformations and peptide-carbohydrate compositions.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• ELISA

Immunohistochemistry: Validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15941 NCBI: NM_002456 NP_002447.4