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Catalog Number Size Price
LS-A6598-50 50 µg (1 mg/ml) $440 
FFAR2 / GPR43 Antibody - Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC‑plus™ Polyclonal Rabbit anti‑Human FFAR2 / GPR43 Antibody (C‑Terminus, IHC) LS‑A6598

IHC‑plus™ Polyclonal Rabbit anti‑Human FFAR2 / GPR43 Antibody (C‑Terminus, IHC) LS‑A6598

Antibody:
FFAR2 / GPR43 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-A6598-50

Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
FFAR2 / GPR43 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GPR43 antibody LS-A6598 is an unconjugated rabbit polyclonal antibody to human GPR43 (FFAR2) (C-Terminus). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human FFAR2 / GPR43
Synonyms
FFAR2 | FFA2R | Free fatty acid receptor 2 | G protein-coupled receptor 43 | G-protein coupled receptor 43 | LSSIG | GPR43 | Gpcr43
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 16 amino acid peptide from C-Terminus of human GPR43. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Monkey (94%).
Epitope
C-Terminus
Specificity
Human GPR43. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except BMI1 (50%).
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 14 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FFAR2 / GPR43
O15552 NM_005306 NP_005297.1

Validation

FFAR2 / GPR43 Antibody - Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR43 antibody IHC of human lung, alveolar macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ FFAR2 / GPR43 Antibody (C-Terminus) for IHC, ELISA LS-A6598 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

A novel antiinflammatory role for the short-chain fatty acids in human labor. Voltolini C, Battersby S, Etherington SL, Petraglia F, Norman JE, Jabbour HN. Endocrinology. 2012 Jan;153:395-403.

Customer Reviews (0)


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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/24/2024