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Catalog Number	Size	Price
LS-B7450-50	50 µg (1 mg/ml)	$460

HSPB1 / HSP27 Antibody - Anti-HSPB1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

HSPB1 / HSP27 Antibody - Hsp27(5D12-A3), HeLa lines. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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IHC‑plus™ Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (clone 5D12‑A12, IHC, WB) LS‑B7450

Name: IHC-plus™ HSPB1 / HSP27 Monoclonal Antibody Mouse anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B7450-50
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C108985

Antibody:

HSPB1 / HSP27 Mouse anti-Human Monoclonal (5D12-A12) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

$460

LS-B7450-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

HSPB1 / HSP27 Mouse anti-Human Monoclonal (5D12-A12) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Other formats:

Specifications

Description

HSP27 antibody LS-B7450 is an unconjugated mouse monoclonal antibody to human HSP27 (HSPB1). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human HSPB1 / HSP27

Synonyms

See All HSPB1 / HSP27 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG2b,k Monoclonal

Clone

5D12-A12

Conjugations

Unconjugated. Also available conjugated with Atto 390, Atto 594, Atto 488, APC, Biotin, FITC, PerCP, RPE.

Purification

Protein G purified from tissue culture supernatant

Modifications

Unmodified

Immunogen

Human Hsp27.

Specificity

Detects a 27kD protein corresponding to the molecular mass of hsp27 on SDS PAGE immunoblots. There is no cross-reactivity to alphaBcrystallin.

Applications

IHC
IHC - Paraffin (10 µg/ml)
Western blot (0.25 - 0.5 µg/ml)
Immunoprecipitation
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About HSPB1 / HSP27

Uniprot: P04792 NCBI: NM_001540 NP_001531.1

Validation

Anti-HSPB1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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About LSBio Validation process