Human IL7R / CD127

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Mouse, Rat (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antigen affinity chromatography

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of mouse IL-7 receptor protein.

This antibody is specific for mouse IL-7 receptor protein. A BLAST analysis was used to suggest cross-reactivity with IL-7 receptor protein from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7 receptor protein from other sources has not been determined. Anti-IL-7 receptor alpha chain antibody is useful for researchers interested in Immunology Research.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:1000 - 1:10000)
• ELISA (1:5000 - 1:50000)

Immunohistochemistry: LS-B2830 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2830 was determined to be 5 ug/ml. Western Blot: Expect a band approximately 55 kD in size corresponding to IL-7 in the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16871 NCBI: NM_002185 NP_002176.2