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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2407-50 | 50 µg (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human Insulin Antibody (clone 8E2, HRP, IHC) LS‑B2407
IHC‑plus™ Monoclonal Mouse anti‑Human Insulin Antibody (clone 8E2, HRP, IHC) LS‑B2407
Note: This antibody replaces LS-C24662
Antibody:
Insulin Mouse anti-Human Monoclonal (HRP) (8E2) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Insulin Mouse anti-Human Monoclonal (HRP) (8E2) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Specifications
Description
Insulin antibody LS-B2407 is an HRP-conjugated mouse monoclonal antibody to human Insulin. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human Insulin
Synonyms
INS | IRDN | IDDM2 | Preproinsulin | ILPR | Insulin | MODY10 | Proinsulin
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
8E2
Conjugations
Horseradish Peroxidase
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Purified human insulin.
Specificity
Recognizes human Insulin. Species cross-reactivity: human proinsulin, bovine insulin (30%) and porcine insulin. Does not cross-react with free C-peptide.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2407 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2407 was determined to be 10 ug/ml. Recommended as a detection antibody for ELISA and in sandwich assay with clone 3A6 as the capture antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.05% ProClin™ 300
Storage
Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Insulin
Validation
Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ Insulin Antibody (clone 8E2, HRP) for IHC, ELISA LS-B2407 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Flow Cytometry
Species:
Rat, Human, Mouse, Bovine, Pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA, Dot Blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
