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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2443-125 | 125 µl (0.2 mg/ml) | $525 |
IHC‑plus™ Monoclonal Mouse anti‑Human KRT14 / CK14 / Cytokeratin 14 Antibody (C‑Terminus, IHC, WB) LS‑B2443
IHC‑plus™ Monoclonal Mouse anti‑Human KRT14 / CK14 / Cytokeratin 14 Antibody (C‑Terminus, IHC, WB) LS‑B2443
Note: This antibody replaces LS-C24972
Antibody:
KRT14 / CK14 / Cytokeratin 14 Mouse anti-Human Monoclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
KRT14 / CK14 / Cytokeratin 14 Mouse anti-Human Monoclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
Cytokeratin 14 antibody LS-B2443 is an unconjugated mouse monoclonal antibody to Cytokeratin 14 (KRT14 / CK14) (C-Terminus) from human. It is reactive with human and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KRT14 / CK14 / Cytokeratin 14
Synonyms
KRT14 | CK-14 | EBS3 | EBS4 | K14 | Keratin-14 | CK14 | Cytokeratin 14 | Cytokeratin-14 | Keratin 14 | NFJ
Host
Mouse
Reactivity
Human, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG3
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A 15 AA synthetic peptide corresponding to the C-Terminus of human keratin 14. Cellular Localization: Cytoplasmic.
Epitope
C-Terminus
Specificity
Recognizes human keratin 14 at 50kD. Species cross-reactivity: Rat.
Applications
- IHC
- IHC - Paraffin (1:50)
- Western blot (1 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2443 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2443 was determined to be 1:50. Positive control: A431 cells, skin or squamous cell carcinoma.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
About KRT14 / CK14 / Cytokeratin 14
Validation
Anti-KRT14 / Cytokeratin 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-KRT14 / Cytokeratin 14 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
See More About...
LSBio Ratings
IHC-plus™ KRT14 / CK14 / Cytokeratin 14 Antibody (C-Terminus) for IHC, WB/Western LS-B2443 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
Immunofluorescence, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin
Reactivity:
Human
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
