Target
Human KRT14 / CK14 / Cytokeratin 14
Synonyms
KRT14 | CK-14 | EBS3 | EBS4 | K14 | Keratin-14 | CK14 | Cytokeratin 14 | Cytokeratin-14 | Keratin 14 | NFJ
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG3
Monoclonal
Purification
Ion exchange chromatography
Immunogen
Synthetic peptide corresponding to the last 15 residues of extreme C-Terminus of human keratin 14 (Thyroglobulin coupled).
Specificity
Recognizes human Cytokeratin 14.
Applications
- IHC
- IHC - Paraffin (1:50 - 1:100)
- IHC - Frozen
- Flow Cytometry
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Usage
Suitable for use in Flow Cytometry and Immunohistochemistry. Flow Cytometry: 10 ul per test. Immunohistochemistry (Formalin fixed paraffin sections or frozen tissues): Recommended to either microwave treat fixed tissue sections or use high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use is neutral buffered formalin. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% Sodium Azide, 1% BSA
Storage
Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT14 / CK14 / Cytokeratin 14