IHC‑plus™ Monoclonal Mouse anti‑Human KRT8 / CK8 / Cytokeratin 8 Antibody (clone C‑51, Concentrated, IHC, WB) LS‑B5555
IHC‑plus™ Monoclonal Mouse anti‑Human KRT8 / CK8 / Cytokeratin 8 Antibody (clone C‑51, Concentrated, IHC, WB) LS‑B5555
Note: This antibody replaces LS-C134823
Antibody:
KRT8 / CK8 / Cytokeratin 8 Mouse anti-Human Monoclonal (Concentrated) (C-51) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Format:
Unconjugated, Concentrated
Overview
Antibody:
KRT8 / CK8 / Cytokeratin 8 Mouse anti-Human Monoclonal (Concentrated) (C-51) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Format:
Unconjugated, Concentrated
Specifications
Description
Cytokeratin 8 antibody LS-B5555 is an unconjugated mouse monoclonal antibody to human Cytokeratin 8 (KRT8 / CK8). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KRT8 / CK8 / Cytokeratin 8
Synonyms
KRT8 | CARD2 | CK-8 | CYK8 | Cytokeratin-8 | CK8 | K2C8 | K8 | KO | Keratin-8 | Type-II keratin Kb8 | Cytokeratin 8 | Keratin 8
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Modifications
Concentrated
Specificity
For frozen and paraffin embedded tissues the optimal dilutions have to be determined for use within each laboratory.
Applications
- IHC
- IHC - Paraffin (1:50)
- IHC - Frozen
- Western blot
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
In immunochemistry on cultured cells it strongly reacts with methanol/acetone fixed cells of epithelial origin. In frozen section this antibody reacts with all simple epithelia derived structures). In paraffin embedded tissues a FKFM-fixation (40% formalin, acetic acid and distilled water 2:1:17 with 8.5 g NaCl/L) should be used. Methacarn and formalin fixatives produce less satisfactory results. A protease digestion is beneficial for the result. This antibody can also be used in Western blotting.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT8 / CK8 / Cytokeratin 8
Validation
Anti-KRT8 / Cytokeratin 8 antibody IHC of human liver, bile duct. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Anti-KRT8 / Cytokeratin 8 antibody IHC of human liver, bile duct. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human, Bovine, Rabbit, Sheep, Porcine
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Bovine, Guinea pig, Hamster, Pig
Applications:
IHC, Western blot
Species:
Human, Rat, Rabbit
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024