Human NES / Nestin

Rabbit

Human, Mouse (tested or 100% immunogen sequence identity)

Human, Rabbit, Macaque (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.

A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and macaque (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from rabbit (94%), mouse (reactivity shown above), rat and hamster (88%), and bovine and dog (82%). Reactivity against homologues from other sources is not known.

• IHC (5 µg/ml)
• IHC - Paraffin (5 µg/ml)
• Western blot (1:500 - 1:3000)
• ELISA (1:20000 - 1:80000)

Immunohistochemistry: LS-B656 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B656 was determined to be 5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P48681 NCBI: X65964 CAA46780.1