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Catalog Number Size Price
LS-B15551-50 50 µl $460 
PCNA Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
PCNA Antibody - Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
PCNA Antibody - Immunohistochemistry of paraffin-embedded mouse lung tissue, at a dilution of 1:200.
PCNA Antibody - Immunohistochemistry of paraffin-embedded human gastric tissue, at a dilution of 1:200.
PCNA Antibody - Immunohistochemistry of paraffin-embedded human colon tissue, at a dilution of 1:200.
PCNA Antibody - Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
PCNA Antibody - Western blot analysis of extracts of various cell lines, using PCNA antibody.
PCNA Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
PCNA Antibody - Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
PCNA Antibody - Immunohistochemistry of paraffin-embedded mouse lung tissue, at a dilution of 1:200.
PCNA Antibody - Immunohistochemistry of paraffin-embedded human gastric tissue, at a dilution of 1:200.
PCNA Antibody - Immunohistochemistry of paraffin-embedded human colon tissue, at a dilution of 1:200.
PCNA Antibody - Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
PCNA Antibody - Western blot analysis of extracts of various cell lines, using PCNA antibody.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PCNA Antibody (IHC, WB) LS‑B15551

IHC‑plus™ Polyclonal Rabbit anti‑Human PCNA Antibody (IHC, WB) LS‑B15551

Note: This antibody replaces LS-C330885
Antibody:
PCNA Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B15551-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PCNA Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PCNA antibody LS-B15551 is an unconjugated rabbit polyclonal antibody to PCNA from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PCNA
Synonyms
PCNA | Cyclin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1). MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS
Specificity
Human PCNA / Cyclin
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 28kDa, while the observed MW by Western blot was 36kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PCNA
P12004 NM_002592 NP_002583.1

Validation

PCNA Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.

PCNA Antibody - Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.
Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.

LSBio Ratings

IHC-plus™ PCNA Antibody for IHC, WB/Western LS-B15551 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024