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Catalog Number Size Price
LS-B540-0.05 0.05 mg (1 mg/ml) $460 
PDCD1 / CD279 / PD-1 Antibody - Anti-PD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PDCD1 / CD279 / PD-1 Antibody - Immunofluorescence of PD-1 in Human Brain cells with PD-1 antibody at 20 ug/ml.
PDCD1 / CD279 / PD-1 Antibody - Western blot of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
PDCD1 / CD279 / PD-1 Antibody - Anti-PD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PDCD1 / CD279 / PD-1 Antibody - Immunofluorescence of PD-1 in Human Brain cells with PD-1 antibody at 20 ug/ml.
PDCD1 / CD279 / PD-1 Antibody - Western blot of PD-1 in THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PDCD1 / CD279 / PD‑1 Antibody (IHC, IF, WB) LS‑B540

IHC‑plus™ Polyclonal Rabbit anti‑Human PDCD1 / CD279 / PD‑1 Antibody (IHC, IF, WB) LS‑B540

Note: This antibody replaces LS-C19688
Antibody:
PDCD1 / CD279 / PD-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B540-0.05
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PDCD1 / CD279 / PD-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PD-1 antibody LS-B540 is an unconjugated rabbit polyclonal antibody to PD-1 (PDCD1 / CD279) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human PDCD1 / CD279 / PD-1
Synonyms
PDCD1 | CD279 | HPD-1 | HPD-l | PD1 | Protein PD-1 | SLEB2 | PD-1 | CD279 antigen | Programmed cell death 1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Immunogen
A 16 AA synthetic peptide near the C-Terminus of human PD-1. The immunogen is located within aa 210-260 of PD-1.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
PD-1 antibody can be used for detection of PD-1 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 45 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD1 / CD279 / PD-1
Q15116 NM_005018 NP_005009.2

Validation

PDCD1 / CD279 / PD-1 Antibody - Anti-PD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ PDCD1 / CD279 / PD-1 Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B540 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Somatic Mutations and Immune Alternation in Rectal Cancer Following Neoadjuvant Chemoradiotherapy. Dengbo Ji , Haizhao Yi , Dakui Zhang , Tiancheng Zhan, Zhaowei Li, Ming Li, Jinying Jia, Meng Qiao, Jinhong Xia, Zhiwei Zhai, Can Song, Jin Gu. Cancer immunology research. 2018 Nov;6:1401-1416.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/4/2024