Human SDF1 / CXCL12

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with Biotin.

Immunoaffinity purified

Unmodified

E.coli derived recombinant Human SDF-1alpha.

Human SDF-1alpha

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 2 µg/ml)

Immunohistochemistry: LS-B7489 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 2.5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human SDF-1alpha (100 ng/ml), a concentration of 2-4 ug/ml of this antibody is required. ELISA: To detect Human SDF-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SDF-1alpha (LS-C104650) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human SDF-1alpha. Western Blot: To detect Human SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Lyophilized from PBS, pH 7.2

Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P48061 NCBI: NM_000609 NP_000600.1