Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Murine SDF-1alpha (100 ng/ml), a concentration of 2.5-3.5 ug/ml of this antibody is required. ELISA: To detect Murine SDF-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine SDF-1alpha (
LS-C104407) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine SDF-1alpha. Western Blot: To detect Murine SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine SDF-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.