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| Catalog Number | Size | Price |
|---|---|---|
| LS-B421-50 | 50 µg (1 mg/ml) | $775 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (IHC, WB) LS‑B421
IHC‑plus™ Polyclonal Rabbit anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (IHC, WB) LS‑B421
Note: This antibody replaces LS-C18950
Antibody:
SERPINA1 / Alpha 1 Antitrypsin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
SERPINA1 / Alpha 1 Antitrypsin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Alpha 1 Antitrypsin antibody LS-B421 is an unconjugated rabbit polyclonal antibody to human Alpha 1 Antitrypsin (SERPINA1). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SERPINA1 / Alpha 1 Antitrypsin
Synonyms
SERPINA1 | A1A | A1AT | AAT | Alpha 1 Antitrypsin | Alpha-1 protease inhibitor | Alpha-1-antiproteinase | Alpha-1-antitrypsin | Alpha1AT | PRO2275 | Alpha-1-antitrypsin null | PI1 | Serpin A1 | Alpha 1 AT
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Human Alpha-1-anti-Trypsin.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:2000 - 1:10000)
- ELISA (1:10000 - 1:600000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B421 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B421 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SERPINA1 / Alpha 1 Antitrypsin
Validation
Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ SERPINA1 / Alpha 1 Antitrypsin Antibody for IHC, WB/Western, ELISA LS-B421 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human, Monkey, Dog
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Monkey, Dog
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Monkey, Dog
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Monkey, Horse
Applications:
IHC, IHC - Paraffin
Reactivity:
Rat
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
